CyFlow™ CD3 act. Purified
| Antigen: | CD3 (activation epitope) |
| Clone: | APA1/1 |
| Application: | Flow cytometry, Immunocytochemistry, Immunohistochemistry (frozen sections), Immunoprecipitation, Western blot |
| Format/Fluorochrome: | Purified |
| Target Species: | Human, Mouse |
| Field of Interest: | Immunophenotyping |
| Species of Origin: | Mouse |
| Regulatory Status: | RUO |
| Clonality: | monoclonal |
| Isotype: | IgG1 |
| HLDA Workshop | HLDA III—WS Code T 126 |
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 |
| Immunogen | Purified human CD3 proteins isolated from thymus |
| Background Information | CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3 δ, CD3ε and CD3ζ. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Alarcón B, Ley SC, Sánchez-Madrid F, Blumberg RS, Ju ST, Fresno M, Terhorst C: The CD3‑gamma and CD3‑delta subunits of the T cell antigen receptor can be expressed within distinct functional TCR/CD3 complexes. EMBO·J. 1991·Apr; 10(4):903‑12. <·PMID:·1826255·> | Delgado P, Fernández E, Dave V, Kappes D, Alarcón B: CD3delta couples T‑cell receptor signalling to ERK activation and thymocyte positive selection. Nature. 2000·Jul·27; 406(6794):426‑30. <·PMID:·10935641·> | Torres PS, Alcover A, Zapata DA, Arnaud J, Pacheco A, Martín-Fernández JM, Villasevil EM, Sanal O, Regueiro JR: TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J·Immunol. 2003·Jun·15; 170(12):5947‑55. <·PMID:·12794121·> | Gil D, Schrum AG, Alarcón B, Palmer E: T cell receptor engagement by peptide‑MHC ligands induces a conformational change in the CD3 complex of thymocytes. J·Exp·Med. 2005·Feb·21; 201(4):517‑22. <·PMID:·15728235·> | Risueño RM, Gil D, Fernández E, Sánchez-Madrid F, Alarcón B: Ligand‑induced conformational change in the T‑cell receptor associated with productive immune synapses. Blood. 2005·Jul·15; 106(2):601‑8. <·PMID:·15790785·> | Rieux-Laucat F, Hivroz C, Lim A, Mateo V, Pellier I, Selz F, Fischer A, Le Deist F: Inherited and somatic CD3zeta mutations in a patient with T‑cell deficiency. N·Engl·J·Med. 2006·May·4; 354(18):1913‑21. <·PMID:·16672702·> | Risueño RM, van Santen HM, Alarcón B: A conformational change senses the strength of T cell receptor‑ligand interaction during thymic selection. Proc·Natl·Acad·Sci·USA. 2006·Jun·20; 103(25):9625‑30. <·PMID:·16766661·> | Wong AP, Dutly AE, Sacher A, Lee H, Hwang DM, Liu M, Keshavjee S, Hu J, Waddell TK: Targeted cell replacement with bone marrow cells for airway epithelial regeneration. Am·J·Physiol·Lung·Cell·Mol·Physiol. 2007·Sep; 293(3):L740‑52. <·PMID:·17616650·> | Tailor P, Tsai S, Shameli A, Serra P, Wang J, Robbins S, Nagata M, Szymczak-Workman AL, Vignali DA, Santamaria P: The proline‑rich sequence of CD3epsilon as an amplifier of low‑avidity TCR signaling. J·Immunol. 2008·Jul·1; 181(1):243‑55. <·PMID:·18566390·>
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